GTPase NRas anticorps (Internal Region)

N° du produit ABIN375000

Cet anticorps anti-GTPase NRas est un anticorps Chèvre Polyclonal détectant GTPase NRas dans WB et IHC (p). Adapté pour Humain, Rat, Souris et Chien.

Aperçu rapide pour GTPase NRas anticorps (Internal Region) (ABIN375000)

Antigène: GTPase NRas (NRAS)

Reactivité: Humain, Rat, Souris, Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp GTPase NRas est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-GTPase NRas anticorps

Épitope: Internal Region

Séquence: C-RMKKLNSSDD GTQ

Specificité: This antibody detects NRAS.

Réactivité croisée (Details): Species reactivity (expected):Mouse, Rat and Canine (Dog).
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Synthetic peptide corresponding to internal sequence of Human NRAS (NP_002515.1). Genename: NRAS

Information d'application

Indications d'application: Peptide ELISA: Antibody detection limit dilution 1/32000. Western Blot: 0.01-0.03 μg/mL. Approx.21 kDa band observed in Human lysates of cell lineA431. In transfected HEK293 transiently expressing NRAS bands of approx.21 kDa and21 kDa are observed. These bands are not observed in the non-transfected HEK293. Immunohistochemistry: 2-3 μg/mL. In paraffin embedded Human Small Intestine shows stainingof cytoplasm and membrane of enterocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur GTPase NRas

Antigène: GTPase NRas (NRAS)

Autre désignation: NRAS

Sujet: NRAS is an oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.Synonyms: ALPS4, GTPase NRas, HRAS1, N-Ras, Transforming protein N-Ras, neuroblastoma RAS viral (v-ras) oncogene homolog

Poids moléculaire: 21 kDa (NP_002515.1).

ID gène: 4893, 9606

UniProt: P01111

Pathways: Signalisation p53, Signalisation MAPK, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling